anti rhbdd2  (OriGene)

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Rhomboid family gene expression profiling in breast normal tissue and tumor samples.

doi: 10.1007/s13277-013-1199-8

Figure Lengend Snippet: Fig. 4 RHBDD2 protein expression analysis in breast cancer samples. a Percentage of negative and positive cases for RHBDD2 expression according to ER/PR status and tumor stages. b Immunohistochemical staining for RHBDD2 protein in breast carcinomas showing negative (Tumor 1) and positive reactions (Tumor 2 and Tumor 3). The image magnification is ×400, and for the upper right inset is ×1,000. Arrows indicate brown positive staining localized in the perinuclear area of the cells. Scale bar=20 μm. c Western blot analysis of RHBDD2 in a positive control derived from RHBDD2 HEK293T overexpression lysate (Ctr.), MCF7, T47D, KB (derived from HeLa) cell lines and the three primary breast carcinomas previously analyzed by IHC (T1, T2, T3). WM molecular weight marker

Article Snippet: RHBDD2 protein was detected using a commercially anti-RHBDD2 polyclonal antibody (TA306891, Origene, USA) and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Dako, Denmark, 1:2,000) followed by enhanced chemiluminescence (ECL).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot, Positive Control, Derivative Assay, Over Expression, Molecular Weight, Marker